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. 2014 Apr 28;205(2):155–162. doi: 10.1083/jcb.201309029

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© 2014 Lesman et al.

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Figure 4. — Dividing cells embedded in fluorescently labeled fibrin matrices. (A) Fixed gels showing a dividing fibroblast (GFP-actin, green) and the fibrin matrix (red) during cytokinesis. Dividing cells deform the matrix locally around the mitotic protrusions (strong red signal due to dense fibers). Matrix voids are evident between the mitotic cell body and the matrix (“2D slice,” arrowheads). (B) One confocal plane of a live dividing cell (actin-GFP, green) and the labeled fibrin matrix (gray) at cell rounding (t = 0), cytokinesis (t = 6 min), and shortly after division (t = 54 min). See Video 10 and Fig. S3 for the entire sequence of events. Bars, 30 µm.