Figure 3.

Cells morphous and immunocytochemical characterization of the co-culture units. (a) Cells morphous of co-culture units U937-HPMC-ESC, HPMC and ESC-HPMC were observed by using inverted microscope (×100). (b) Immunocytochemistry shows that HPMCs express both vimentin and cytokeratin, and ESCs express vimentin but do not express cytokeratin. The HPMCs are stained by anti-vimentin mAb and anti-cytokeratin mAb with isotypic control. The ESC-HPMC co-culture is stained by anti-vimentin mAb, anti-cytokeratin mAb and isotypic control (×200). ESC, endometriotic stromal cell; HPMC, human peritoneal mesothelial cell; mAb, monoclonal antibodies.