Table 1. Primer pairs for RT-PCR.
| Gene | Primers | Product (bp) |
|---|---|---|
| β-actin | For 5'-TTGCCGACAGGATGCAGAA-3' | 101 |
| Rev 5'-GCCGATCCACACGGAGTACT-3' | ||
| Adenosine A1 receptor | For 5'-GTTCACAGTTTTTTATTAGTCAC-3' | 109 |
| Rev 5'-AACATGAGTGTCAACTCC-3' | ||
| Adenosine A2a receptor | For 5'-CTTGGGTTCTGAGGAAGCAG-3' | 253 |
| Rev 5'-CAGCAGCTCCTGAACCCTAG-3' | ||
| Adenosine A2b receptor | For 5'-ATCTCCAGGTATCTTCTC-3' | 322 |
| Rev 5'-GTTGGCATAATCCACACAG-3' | ||
| Adenosine A3 receptor | For 5'-CTTGATTACTTCCACTGAGGTGG-3' | 334 |
| Rev 5'-CAACATCTTCTAGGCATCCTCC-3' |
Abbreviation: RT-PCR, reverse transcription polymerase chain reaction.