Effects of IL-1β, IL-18 and IL-33 on the viability of human eosinophils. Eosinophils (5×105 per well) were cultured with or without (a) IL-1β, (b) IL-18 or (c) IL-33 (10–100 ng/ml) for 48 h in a 24-well plate. Percentage of viability of eosinophils was assessed by the TACS Annexin V-FITC assay using flow cytometry. *P<0.05, **P<0.01 when compared with medium control. FITC, fluorescein isothiocyanate; IL, interleukin.