Figure 6.
Activation of ERK, p38 MAPK, NF-κB in human eosinophils upon IL-1β, IL-18 and IL-33 stimulation. Eosinophils (5×105 cells) were cultured with or without IL-1β, IL-18 or IL-33 (50 ng/ml) for 15 min. After fixation and permeabilization, the intracellular contents of phosphorylated (a) ERK, (b) p38 MAPK and (c) IκB-α in 10 000 permeabilized eosinophils were measured by intracellular staining and flow cytometry. Results were normalized by subtracting the appropriate isotypic control and shown as the arithmetic mean±SEM of three independent experiments. *P<0.05, **P<0.01 when compared with medium control. Ctrl, medium control; ERK, extracellular signal-regulated kinase; IL, interleukin; MAPK, mitogen-activated protein kinase; NF, nuclear factor.