FIG. 4.
Western blot analysis of HIV-1 proteins in viral particles. Each lane contains proteins from 50 ng of p24 equivalent of viral lysates separated on an SDS-12% polyacrylamide gel and probed with anti-HIV-1 human serum. Lane 1, wild-type virus (WT); lanes 2 to 7, various Cys mutant viruses as identified above the lanes. The numbers on the left are molecular masses in kilodaltons, and the labels on the right correspond to positions of viral proteins or their precursors.