FIG. 2.

Transcriptional enhancement mediated by pIX. A549 cells were cotransfected with a pIX expression plasmid and a reporter plasmid containing the luciferase gene under the control of the E1A promoter. Twenty-four hours later, crude protein extracts were prepared and assayed for luciferase activity. The data have been normalized for transfection efficiency relative to a control MCMV-lacZ expression cassette included in the transfection. Shown is a data set representative of at least two independent transfections.