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Growth of Ad vector lacking pIX. A549 cells were infected at an MOI of 1 with AdRP2233 (pIX+), AdRP2234 (pIX−), or AdRP2234 that had been grown on a pIX-complementing cell line for virions which contained pIX (AdRP2234pIX+). At various times postinfection, the medium and cells were collected and their titers were determined on 293 cells.
