Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Expression of late protein from Ad vector with pIX deleted. A549 cells were infected at an MOI of 10 with AdRP2233 or AdRP2234. At various times postinfection (in hours, shown above the gels), the medium was removed and the cells were lysed with SDS-PAGE loading buffer. The protein samples were separated by SDS-10% PAGE, transferred to a PVDF membrane, and immunoblotted with antibody to Ad5 fiber or tubulin. Numbers at left are molecular masses in kilodaltons.
