FIG. 6.

Growth of Ad vector with pIX deleted in 293pIXc4 cells. (A) The level of pIX expression in 293pIXc4 cells was verified by Western blot analysis with 293 cells infected with Ad at various MOIs as a standard. (B) 293pIXc4 cells were infected at an MOI of 1 with AdRP2233 or AdRP2234, the medium and cells were harvested 24 h later, and their titers were determined on 293 cells. (C) Protein samples were prepared in duplicate from cells infected with the indicated virus and analyzed by immunoblotting with fiber- or tubulin-specific antibody.