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. 2010 Dec 6;32(1):89–98. doi: 10.1038/aps.2010.173

Figure 1.

Figure 1

Identification of anti-proliferative and apoptotic effects. (A) Chemical structure of WJ9708012. (B) The graded concentrations of WJ9708012 were added to cells for 48 h. Then, the cells were fixed and stained with SRB and the data were analyzed as described in the Materials and methods section. Data are expressed as mean±SEM of four determinations (each in triplicate). (C and D) PC-3 cells were treated with the indicated agent. Then, the cells were harvested for annexin V-PI double staining of apoptosis or in situ detection of apoptotic cells by using TUNEL apoptosis detection methods. Data are expressed as mean±SEM of three determinations. Scale bar, 20 μm.