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. 2010 Nov 29;32(1):38–44. doi: 10.1038/aps.2010.182

Figure 2.

Figure 2

Sal B decreased the accumulation of LC3-II and LC3-positive fluorescence dots in starving cardiac myocyes. Cells were cultured in GD 3 h in presence (+) or absence (−) of chloroquine (Chlo, 3 μmol/L) or Sal B (50 μmol/L) with cells in CM as control. Cell lysates were subjected to immunoblots for detection of LC3-I, LC3-II, and α-actin. (A) Representative immunoblots of LC3 and the relative amount of LC3-II were shown. Western blotting of α-actin showed equal loading of the samples. (B) Representative images of LC3-positive fluorescence. Green fluorescence presented LC3-FITC staining of autophagosomes. Blue fluorescence indicated DAPI-stained nucleus. Data are mean of three culture preparations. bP<0.05 vs CM, eP<0.05 vs GD.