Skip to main content
. 2010 Oct 18;31(11):1478–1486. doi: 10.1038/aps.2010.100

Figure 2.

Figure 2

Changes in the morphology and ultrastructure of embryonic stem cells during the process of differentiation. (A) Light microscopic comparison of morphologic changes among cells treated with different combinations of sodium butyrate (SB), basic fibroblast growth factor (bFGF), and bone morphogenetic protein 4 (BMP4) in the early stages of differentiation. Spontaneously differentiated cells were used as controls (magnification, 200×). (B) Morphologic changes on day 15 in cells treated with SB, bFGF, and BMP4 seen through a light microscope (magnification, 400×). (C–E) Electron micrographs of day 15 cells treated with SB, bFGF, and BMP4 (magnification: [C] 10 000×, [D] 20 000×, [E] 50 000×). Arrows in (C) and (E) indicate the formation of glycogen pools containing large amounts of glycogen.