Table 1. Information of the analyzed LRP5 SNPs in this study.
SNP | Location | Amino acid change | PCR primer | Restriction enzyme |
---|---|---|---|---|
Q89R (c.314A>G) | Exon 2 | Gln [Q]>Arg [R] | Forward: 5′-TCTGGGCATAGTGCTCCATC-3′ Reverse: 5′-TTCCGGGATGTGCCATTGAG-3′ | Ava II |
N740N (c.2268T>C) | Exon 10 | Asn [N]>Asn [N] | Forward: 5′-CTACTGGGCCGACACTGGGATTAA-3′ Reverse: 5′-ACAGCTCTAATCACTGAGGG-3′ | Ase I |
A1330V (c.4037C>T) | Exon 18 | Ala [A]>Val [V] | Forward: 5′-GACTGTCAGGACCGCTCACACG-3′ Reverse: 5′-AAGGTTTTCAGAGCCCCTAC-3′ | Dra III |