Figure 3.

Perifosine's effect on phosphorylation and protein expression of Akt and JNK. (A) Leukemia cells were exposed to the indicated concentrations of perifosine for 24 h and then analyzed for expression of total and phosphorylated Akt (Ser473). (B) CML and AML cell lines were treated with varying doses of perifosine for 24 h, after which cells were harvested, and extracted proteins were immunoblotted for the expression of total and phosphorylated JNK, and β-actin. These experiments were repeated once with identical results.