Figure 6.

Inhibition of autophagy results in enhanced suppression of cell growth and apoptosis in CML cell lines. (A) K562 and K562/G cells were treated for 48 h with perifosine (PRF, 20 μmol/L), chloroquine (CQ, 40 nmol/L) or two agents combination, respectively. Viability of cells growth was examined by a MTT assay. Mean±SD. n=3. (B) Western blotting analysis was performed for the expression of LC3 and β-actin. (C) The levels of BCR/ABL fusion protein, cleavage of PARP, and activation of caspase-3 were analyzed using western blotting. Anti-β-actin was used as a control for protein loading. ^cP<0.01 vs perifosine.