Figure 7.

Piperine inhibited the metastasis of 4T1 tumors in vivo. 4T1 cells were implanted subcutaneously into the female BALB/c mice. After 3 days of implantation, piperine (0 and 5 mg/kg) was dissolved in 0.2% DMSO and intratumorally injected every three days for three times. Primary tumor was surgically removed on d 15 and mice were sacrificed on d 30 post-tumor inoculation for analysis of lung metastases by colonogenic assay. Mean±SD. n=3. ^bP<0.05.