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. 2013 Nov 4;11(2):197–205. doi: 10.1038/cmi.2013.52

Figure 3.

Figure 3

The Ets-1 transcription factor binds to the Ig Iα1 promoter in vitro and in cells. (a–c) EMSA assays indicate that two different DNA/protein complexes could be observed when incubating the Ets binding site probe with a nuclear extract from Raji, CNE1 or HeLa cells. (d) SuperShift-EMSA assays demonstrate possible Ets-1 transcription factor binding to the Ets site in the Ig Iα1 promoter. (e) ChIP assays confirm that the Ets-1 transcription factor exerts its regulatory function through direct binding to the human Ig Iα1 promoter in cells. ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay; Ig, immunoglobulin.