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. 2013 Dec 9;11(2):132–140. doi: 10.1038/cmi.2013.55

Figure 1.

Figure 1

The TLR4 mutation is accompanied with an increase in pro-B and pre-B cells in the bone marrow. Bone marrow cells from C3H/HeN and TLR4 mutant C3H/HeJ mice were analyzed by flow cytometry following staining with antibodies against B220, Mac-1, CD43 and IgM. (a) Representative dot plots for B220 and Mac-1 staining. (b) The percentage of B220+ and Mac-1+ cells and their absolute numbers harvested from one femur and one tibia. The data are presented as the means±s.d. (n=7–13). (c) Representative dot plots for CD43, B220 and sIgM staining. The number indicates the percentage of the gated population. (d) The absolute numbers of pro-B (B220+CD43+IgM−), pre-B (B220+CD43−IgM−), immature B (B220+CD43−IgM+) and mature B (B220hiCD43−IgM+) cells harvested from one femur and one tibia. The data are presented as the means±s.d. (n=7–13). *P<0.05, **P<0.01, ***P<0.0001, NS: not significant. IgM, immunoglobulin M; TLR4, Toll-like receptor 4.