Figure 3.

LPS promotes differentiation of pre-B cells in IL-7 culture. (a) Purified pre-B (B220⁺CD43⁻IgM⁻IgD⁻) cells were cultured at a density of 2×10⁵/well for 72 h in medium alone or in the presence of IL-7 (10 ng/ml), LPS (10 µg/ml) or both. Cells were then stained for surface expression of IgM and IgD. Dot plots show the staining profile of a representative experiment of four independent experiments. The numbers indicates the percentage of cells in each quadrant. (b) The number of IgM⁺IgD⁻ and IgM⁺IgD⁺ cells generated in the cultures. (c) Similar experiments were performed with pre-B cells from C3H/HeN and C3H/HeJ mice. Dot plots show the results of a representative experiment of three independent experiments. (d) The number of IgM⁺IgD⁺ cells in cultures. *P<0.05, **P<0.01, NS: not significant. IgD, immunoglobulin D; IgM, immunoglobulin M; LPS, lipopolysaccharide.