Figure 9.

Colocalization of SARS coronavirus M protein and its TM1′ mutant with TRAF3. HeLa cells were transfected with plasmids expressing TRAF3 and V5-tagged SARS coronavirus M protein (SCV M), its TM1′ mutant or human coronavirus HKU1 M protein (HKU1 M). Cells were then stained for M and TRAF3. Nuclear morphology (blue) was visualized with DAPI. Specific fluorescent signals from V5 and TRAF3 were then merged. Transfected cells in the merged panels are highlighted by arrows. Colocalization appeared yellow. All panels shown are representative of the results of triplicate experiments. Bar=20 µm. DAPI, 4′,6-diamidino-2-phenylindole; IFN, interferon; SARS, severe acute respiratory syndrome.