Fig. 7.

Polyacrylamide gel electrophoresis (PAGE) under native conditions. The purified protein from two fractions were loaded on a polyacrylamide gel in native conditions (without SDS and β-mercaptoethanol). Four different concentrations (10 μg, 5 μg, 1 μg and 0.1 μg in total) were loaded. The marker (M) is not clearly visible due to the electrophoresis un-denaturing conditions. The smear related to the first fraction may represent protein aggregating and incorrect refolding after the cleaning steps.