Figure 4. High Capsid Filling Slows ATP Tight Binding and Induces Long-Lived Pauses.

(A) The chemical cycle of each φ29 ATPase subunit.

(B) V_max (blue) and K_M (green) versus capsid filling.

(C) V_max/K_M versus capsid filling.

(D) Mean dwell duration versus capsid filling at saturating [ATP].

(E) Apparent number of rate-limiting kinetic events during the dwell versus capsid filling.

(F) Sample packaging traces at various filling levels highlighting regular dwells and long-lived pauses (LLPs).

(G) Sample traces highlighting the locations of LLPs. For clarity only LLPs longer than 5 s are shown. LLPs from different packaging complexes are colored green, blue, black, and red.

(H) Mean LLP duration versus capsid filling at saturating [ATP].

(I) Frequency of LLP occurrence versus capsid filling at saturating [ATP].

Error bars represent 95% CI.

See also Figures S3 and S4.