hWASp expression in EBVWAS5-2 cells, a patient-derived WASp-negative B-cell line. (a) EBVWAS5-2 cells were transduced with either the 400EF1αhWASp, 400P500hWASp, 400P1600hWASp, or 400MNDhWASp vectors at an MOI=7.5, and hWASp expression was determined 21 days post-transduction by Western blotting. (b) EBVWAS5-2 cells were transduced with the 650MNDhWASp or 650EF1αhWASp vector at various MOIs, and hWASp expression was determined 21 days post-transduction by Western blotting. MOI, multiplicity of infection.