Figure 1. ILC lineage tracing in PLZF^GFPcre reporter mice.

a-c, Top rows, expression of eGFP by indicated cell-types of PLZF^GFPcre+/+ mice (filled grey) and WT (open); Bottom rows, YFP expression in radiation chimeras reconstituted with YFP⁻Lin⁻Sca-1⁺cKit⁺ (LSK) bone marrow cells from PLZF^GFPcre+/− ROSA26-YFP mice. CLP and iILC2 from bone marrow (BM); B & T and NK from spleen; NKT from thymus (eGFP), divided in early stage 1-2 and late stage 3, and from spleen (YFP); ILC2 from lung; DX5⁺ and DX5⁻ NK cells from liver; ILC1 from intestinal IEL; LTi and ILC3 from LPL. BM iILC2 and lung ILC2 were identified as Lin⁻CD25⁺IL-7Rα⁺Thy1.2⁺; LPL ILC2 as CD3ε⁻CD19⁻CD25⁺KLRG1⁺; LPL NCR⁺ ILC3 as CD3ε⁻CD19⁻NKp46⁺NK1.1⁻; LPL CD4⁻ LTi cells as CD3ε⁻CD19⁻IL-7Rα⁺KLRG1⁻CCR6⁺CD4⁻; LPL CD4⁺ LTi cells as CD3ε⁻CD19⁻CCR6⁺CD4⁺; and IEL ILC1 as CD3ε⁻CD19⁻NKp46⁺NK1.1⁺CD160⁺. FACS identification of the remaining subsets is defined in the methods section. d, summary of results (mean ± s.e.m). Data representative of 3-9 individual mice analyzed in at least 2 independent experiments.