Fig. 1.

TbGT8 is involved in N-linked glycan synthesis in the bloodstream form of Trypanosoma brucei. The whole parasite lysates in RIPA (−) were subjected to SDS–PAGE, transferred to PVDF membranes, and detected with tomato lectin. Lanes 1–3 are the lysates of wild type, cKO under non-permissive, and cKO under permissive conditions, respectively. The loss of TbGT8 expression selectively reduced the smear signal at approximately 110 kDa. PNGase F treatment eliminated the lectin binding.