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Expression and N-linked glycosylation of TbGT8HA. Lanes 1–3 are the samples from wild type, cKO under non-permissive, and cKO under permissive conditions, respectively. Panel A: Immunoblot detection with an anti-HA antibody. Panel B: CBB staining of the same membrane as the loading control. Arrowheads indicate the position of TbGT8HA. De-N-glycosylation resulted in a decrease in the molecular weight.
