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. Author manuscript; available in PMC: 2014 Apr 29.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Oct 10;33(12):2771–2779. doi: 10.1161/ATVBAHA.113.302571

Figure 1.

Figure 1

Id3−/−Apoe−/− mice have fewer B-1a B cells in the spleen and peritoneal cavity (PerC) compared with Id3+/+Apoe−/− mice. A, Number of B-2 B cells (CD19+B220hi) and B-1a (CD19+B220loCD5+CD43+IgMhi) in the spleen and PerC of Id3+/+Apoe−/− (n=13) or Id3−/−Apoe−/− (n=12) mice at 8 weeks as measured by flow cytometry. B, E06 levels in the serum of Id3+/+Apoe−/− or Id3−/−Apoe−/− mice as measured by ELISA. C, E06 sIgM transcript levels in peritoneal B cell subsets, B-1a (CD19+B220lo CD5+) and B-2 (CD19+B220hi) B cells, measured by real-time polymerase chain reaction and normalized to total sIgM. D, Bromodeoxyuridine (BrdU) incorporation and annexin V staining in PerC B cell subsets of Id3+/+Apoe−/− (n=6 or 7) or Id3−/−Apoe−/− (n=6 or 7) mice from 3 independent experiments. *P<0.05, **P<0.01, and ***P<0.001.