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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Dec 31;1838(5):1389–1395. doi: 10.1016/j.bbamem.2013.12.018

Fig. 3. Saturation transfer difference (STD) spectra of the α7 TM domain for halothane binding.

Fig. 3

(A) Prolonged saturation time increased halothane (3.2 mM) signal in the STD spectra in the presence of α7. The STD spectra resulted from the subtraction of the off- (25 ppm; blank region) from the on-resonance (0.4 ppm; protein methyl group) spectra. (B) STD amplification (%) as a function of the saturation time. STD amplification is defined as (Voff − Von)/Voff, Voff and Von are the integrals of halothane peak in the spectra with off- and on-resonance saturation, respectively.