TABLE 2.
Relative amounts of viral DNA (expressed as the ratios of VP5 DNA to actin DNA) at a high dose of inoculation (500,000 PFU)a
| dpi | Mean value ± SEM in corneas for:
|
Mean value ± SEM in ganglia for:
|
||
|---|---|---|---|---|
| 17ΔPst | 17ΔPstR | 17ΔPst | 17ΔPstR | |
| 1 | 1.51 ± 0.54 | 1.19 ± 0.99 | 0.12 ± 0.12 | 0.23 ± 0.19 |
| 2 | 2.29 ± 0.76 | 1.40 ± 0.94 | 0.65 ± 0.26 | 0.37 ± 0.28 |
| 3 | 2.11 ± 0.32 | 2.38 ± 0.59 | 1.10 ± 0.26 | 1.86 ± 0.66 |
| 5 | 2.31 ± 0.64 | 1.59 ± 0.18 | 1.80 ± 0.36 | 1.74 ± 0.39 |
| 7 | 2.16 ± 1.30 | 2.01 ± 0.27 | 0.80 ± 0.20 | 0.54 ± 0.40 |
| 14 | 0.44 ± 0.14 | 0.36 ± 0.34 | 0.43 ± 0.30 | 0.21 ± 0.23 |
a
Rabbits' eyes were inoculated with 500,000 PFU of 17ΔPst or 17ΔPstR (rescue strain). At the indicated times postinfection, the rabbits (two rabbits per virus per time point) were sacrificed and corneas (four per virus per time point) and TG (four per virus per time point) were dissected. Total DNA was isolated from the tissue and amplified with VP5 and actin gene primer sets in combination. The relative amounts of viral DNA (ratios of VP5 DNA to actin DNA) were determined by densitometry.