FIG. 8.

Replication of SINwt variants containing different adaptive mutations in genomes in C₇10 and BHK-21 cells. (A) Schematic representation of viral genomes, infectivities of in vitro-synthesized RNAs in C₇10 and BHK-21 cells in the infectious center assay, and sizes of plaques formed on both cell lines. Filled boxes indicate the positions of the introduced mutations found in SIN43 variants adapted for growth in C₇10 cells. (B) Single-step viral growth curves after electroporation of 1 μg of in vitro-synthesized RNAs into C₇10 cells. At the indicated times, the medium was replaced and virus titers were determined on BHK-21 cells, as described in Materials and Methods.