FIG. 3.

Acquisition of endoglycosidase H resistance by wt and mutant proteins. BHK cells transfected with plasmids encoding wt or mutant G proteins were labeled with [³⁵S]methionine and then incubated in chase medium with nonradioactive methionine for the indicated number of minutes (A and B). Cell lysates were immunoprecipitated with polyclonal anti-VSV G, and samples were incubated in the presence (+) or absence (−) of endoglycosidase H (Endo H). The kinetics of acquisition of endoglycosidase H resistance of the processed glycoproteins (A) were quantitated using ImageQuant software (C). The percentage of resistance is expressed as the percent of processed G protein relative to the total G protein detected.