FIG. 2.

Analysis of reverse transcription in infected monocytes and differentiated macrophages. At daily intervals following initiation of monocyte cultures in MCSF, cells were removed and infected with a VSV-G-pseudotyped HIV-1. At 18, 42, and 66 h postinfection, total cellular DNA was isolated and the presence of late reverse transcripts (a) and 2-LTR circle forms of viral cDNA (b) was determined by real-time PCR, while integrated proviruses (c) were visualized by Alu-LTR PCR as described elsewhere (8).