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. 2014 Mar 15;8(3):22–23. doi: 10.7860/JCDR/2014/7957.4094

[Table/Fig-3]:

aGt e7l2 pºcic ftourr e1 5o fm PinCR showing amplification of Aspergillus fumigatus

Aspergillus fumigatus Sequence Product size (bp)
AFUMI CGC CGA AGA CCC CAA CAT GAA CGC ˜385
AFUM2 TAA AGT TGG GTG TCG GCT GGC ˜385
Initial denaturation at 96ºc for 5 minutes
40 cycles Denaturation at 94ºc for 30 seconds
Primer annealing at 58ºc for 30 seconds
Extension at 72ºc for 30 seconds