[Table/Fig-3]:
Aspergillus fumigatus | Sequence | Product size (bp) |
AFUMI | CGC CGA AGA CCC CAA CAT GAA CGC | ˜385 |
AFUM2 | TAA AGT TGG GTG TCG GCT GGC | ˜385 |
Initial denaturation at 96ºc for 5 minutes | ||
40 cycles | Denaturation at 94ºc for 30 seconds | |
Primer annealing at 58ºc for 30 seconds | ||
Extension at 72ºc for 30 seconds |