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Syncytium formation induced by wt AV and L HN and I175E mutated forms of the two proteins in BHK and HeLa cells with different transient expression systems. Panels on the left show BHK cell monolayers, and panels on the right show HeLa cells. Except for the top two monolayers, in which only vector is expressed, cells coexpress wt NDV-AV F with the indicated HN protein. At 22 h posttransfection, the monolayers were fixed with methanol and stained with Giemsa stain.
