TABLE 2.
Mutation of I175E AV-HN at residues 193, 214, 219, and 228 converts its phenotype to that of I175E L-HN
| Strain | Substitution(s) | Result fora:
|
|||
|---|---|---|---|---|---|
| Expression | HAd | NA | Fusion | ||
| AV | I175E | 84.1 ± 12.9 | 0.7 ± 0.9 | NDb | 1.9 ± 0.7 |
| AV | I175E, F193L | 80.5 ± 8.9 | 6.6 ± 1.8 | 0.4 ± 0.3 | 8.9 ± 0.8 |
| AV | I175E, S214T, V219I | 95.6 ± 6.7 | 5.7 ± 1.3 | 0.1 ± 0.1 | 9.1 ± 6.1 |
| AV | I175E, N228S | 77.6 ± 3.7 | 0.6 ± 0.5 | ND | 3.4 ± 0.5 |
| AV | I175E, F193L, S214T, V219I | 79.7 ± 4.2 | 12.8 ± 3.2 | 1.1 ± 0.9 | 28.5 ± 2.1 |
| AV | I175E, F193L, S214T, V219I, N228S | 103.4 ± 2.6 | 53.8 ± 3.8 | 6.0 ± 2.2 | 141.6 ± 11.4 |
| L | I175E | 91.3 ± 8.1 | 54.0 ± 2.8 | 5.6 ± 0.4 | 138.8 ± 12.6 |
a
Data are expressed as percentages of the corresponding wt HN protein. The data for AV and L HN carrying only the I175E substitution are repeated from Table 1 for convenience.
b
ND, none detected.