FIG. 4.

Strategy for localizing the 5′ and 3′ ends of the ORF-E transcript. The DNA sequence of the ORF-E/LR gene region (PstI fragment shown in Fig. 1) and positions of the primers used for the studies in Fig. 5 and 6 are shown. A partial sequence of the adjacent HindIII-L is also presented because it contains putative polyadenylation sites for ORF-E. The ORF-E sense strand is presented. The 3′ terminus of bICP0 and the LR start site (productive infection and latency) are presented to provide landmarks. Solid lines with arrow denote the primers used for strand-specific cDNA synthesis (primers 348 and 487). The dashed lines with arrows denote the positions of the primers used for PCR amplification to localize the 5′ terminus of the ORF-E transcript. The arrows show the directions of the primers. The shaded box indicates the region where the 5′ end is localized. The start codon for ORF-E and the putative protein-coding region of ORF-E are in bold. The positions of the primers used to localize the 3′ end of the ORF-E transcript (1 to 5) are dotted lines with an arrow and are in the directions of the respective primers. Potential polyadenylation signals are in bold and underlined. The nucleotide numbers are those proposed for the LR gene (20).