Figure 6.

Effects of green coffee bean extract and 5-CQA supplementation on the expression of gene involved in inflammation in mice fed a HFD. (a) The expression of TLR2 and TLR4 in epididymal adipose tissue was determined by RT-PCR and normalized to that of GAPDH. (b) Protein levels of p-JNK and JNK in the epididymal adipose tissue were determined by Western blotting. (c) The expression of proinflammatory cytokine genes in the epididymal adipose tissue was determined by RT-PCR and normalized to that of GAPDH. Data represent the results of three independent experiments (n = 2, 3 mice per experiment). P < 0.05 indicates statistical significance. Values are the mean ± SEM, n = 8 for each group.