Table 1.
Primer sequences and PCR conditions.
| Gene description | Primers | Sequences (5′-3′) | T m (°C) | Size (bp) |
|---|---|---|---|---|
| SFRP 5 | F | CTTGGTGTCCTTGCGCTTTA | 61 | 155 |
| R | CTGATGGCCTCATGGAACAG | |||
| DKK2 | F | TCATTCCCTGTTCTTCAGCG | 55 | 144 |
| R | GCATTTCCTTCAGATTGGCA | |||
| WNT10b | F | TTTTGGCCACTCCTCTTCCT | 61 | 183 |
| R | TCCTTTTCCAACCGAAAACC | |||
| Galanin | F | GAGCCTTGATCCTGCACTGA | 60 | 121 |
| R | AGTGGCTGACAGGGTCACAA | |||
| GalR1 | F | CCAAGGGGGTATCCCAGTAA | 60 | 147 |
| R | GGCCAAACACTACCAGCGTA | |||
| GalR2 | F | ATAGTGGTGCTCATGCTGGAA | 60 | 134 |
| R | AGGCTGGATCGAGGGTTCTA | |||
| PKCδ | F | CTGAGCGCTGCAAGAAGAAC | 60 | 146 |
| R | TGGAAACTTTGATCCTGCACTGA | |||
| Cyc-D | F | TGGGAAGTTTTGTTGGGTCA | 55 | 144 |
| R | TCCTTGTCCAGGTAATGCCA | |||
| PPARγ2 | F | TTCGGAATCAGCTCTGTGGA | 55 | 148 |
| R | CCATTGGGTCAGCTCTTGTG | |||
| C/EBPα | F | AAGGCCAAGAAGTCGGTGGA | 55 | 189 |
| R | CCATAGTGGAAGCCTGATGC | |||
| FAS | F | TTGCCCGAGTCAGAGAACC | 55 | 171 |
| R | CGTCCACAATAGCTTCATAGC | |||
| Leptin | F | CTCCAAGGTTGTCCAGGGTT | 55 | 143 |
| R | AAAACTCCCCACACAATGGG | |||
| CD36 | F | ATGACGTGGCAAAGAACAGC | 55 | 160 |
| R | GAAGGCTCAAAGATGCCTCC | |||
| aP2 | F | ACATGAAAGTGGGAGTG | 55 | 128 |
| R | AAGTACTCTCTGACCGGATG | |||
| TLR2 | F | TCTAAAGTCGATCCGCGACAT | 55 | 344 |
| R | TACCCAGCTCGCTCACTACGT | |||
| TLR4 | F | ACCTCTGCCTTCACTACAGA | 48.6 | 223 |
| R | AGGGACTTCTCAACCTTCTC | |||
| TNFα | F | TGTCTCAGCCTCTTCTCATT | 55 | 156 |
| R | AGATGATCTGAGTGTGAGGG | |||
| MCP1 | F | CCAGCAAGATGATCCCAATG | 55 | 450 |
| R | CTTCTTGGGGTCAGCACAGA | |||
| IFNα | F | ATGGCTAGGCTCTGTGCTTTCCT | 58 | 638 |
| R | GGGCTCTCCAGATTTCTGCTCTG | |||
| IFNβ | F | CCACAGCCCTCTCCATCAACTATAAGC | 56 | 372 |
| R | AGCTCTTCAACTGGAGAGCAGTTGAGG | |||
| IL-6 | F | TTGCCTTCTTGGGACTGATG | 55 | 162 |
| R | CCACGATTTCCCAGAGAACA | |||
| GAPDH | F | AGAACATCATCCCTGCATCC | 60 | 321 |
| R | TCCACCACCCTGTTGCTGTA |
SFRP 5: secreted frizzled-related protein 5; DKK2: Dickkopf 2; WNT10b: wingless-type MMTV integration site family, member 10B; GalR1: galanin receptor 1; PKCδ: protein kinase C delta; Cyc-D: cyclin D; PPARγ2: peroxisome proliferator-activated receptor gamma; C/EBPα: CCAAT/enhancer-binding protein, alpha; FAS: fatty acid synthase; CD36: fatty acid translocase; aP2: adipocyte protein2; TLR: Toll-like receptor; TNFα: tumor necrosis factor alpha; IFN: interferon; MCP1: monocyte chemoattractant protein 1; IL-6: interleukin-6; GAPDH: gyceraldehyde-3-phosphate dehydrogenase.