FIG. 8.

Na activates c-Jun transactivator function. HeLa cells were transfected with Gal4-EIB-CAT in conjunction with a vector expressing one of the Gal4-fusion proteins (Gal4-ATF1, Gal4-ATF2, Gal4-CREB, or Gal4-c-Jun) or the control vector expressing Gal4 alone (Gal4). In addition, either pRC-FLAG-BRRF1 (Na) or pRC (vector) was also transfected into the cells. Cells were harvested and assayed for CAT activity at 48 h posttransfection. The data shown represent the results of two independent experiments.