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. 2004 May;78(10):5258–5269. doi: 10.1128/JVI.78.10.5258-5269.2004

FIG. 1.

FIG. 1.

Schematic presentation of NA mutants. Sets of 2 to 5 aa in the NA CT or TMD were replaced with alanines. Chimeric NA TMDs [NA(1T2N)NA and NATRNA] were constructed by swapping either the first 9 aa or the entire NA TMD with that of the TR TMD. ·, amino acids from NA sequences. Amino acids shown in boldface type are from TR sequences. Amino acids in lowercase type are due to created restriction enzyme sites. Note that the TR TMD contains 28 aa instead of the 29 aa of the NA TMD. Numbers above amino acid sequences represent amino acid positions (indicated by ↓) of the WT NA peptide.