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Plaque morphologies of different mutant viruses. MDCK cell monolayers (35-mm-diameter dish) were infected with serially diluted viruses and maintained at different temperatures in agarose overlay medium with (+) or without (−) TPCK-treated trypsin (0.5 μg/ml). At 60 h p.i., the agarose overlay medium was removed, stained for 1 min with crystal violet, and washed with PBS.
