FIG. 8.

Triton X-100 insolubility of different proteins in virions. ³⁵S-labeled virions were prepared as described in the legend to Fig. 6 and treated with 0.1% Triton X-100 on ice for 10 min, soluble (S) and insoluble (I) fractions were immunoprecipitated with the respective antibodies, and proteins were eluted, treated with PNGase F for 3 h, analyzed by SDS-PAGE, autoradiographed, and quantified. The percentage of Triton X-100-insoluble protein was calculated from the results from three to five independent experiments, with an average variation of less than 10%.