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. 2004 May;78(10):5157–5169. doi: 10.1128/JVI.78.10.5157-5169.2004

FIG. 9.

FIG. 9.

Effects of alterations to the C-terminal cytoplasmic tail on interactions with Gag. (A) Virion incorporation of GST fusion proteins containing elongated cytoplasmic tails. 293T cells were cotransfected with pCMVgag and each of the pEBG and pEBG chimeras that encoded elongated cytoplasmic tails as indicated, and cell and virion lysates were analyzed by Western blotting with MAbs 183 and Chessie 8. (B) Incorporation of GST-cytoplasmic subdomain fusion proteins into Gag particles. Cell and virion lysates obtained from 293T cells coexpressing Gag and each of the GST fusion proteins that contained cytoplasmic subdomain fragments as indicated were analyzed by Western blotting with MAbs 183 and Chessie 8. Migration of GST-cytoplasmic tail fusion proteins is marked by GST/CD.