Table 2.
The strains and plasmid used in this study
| Strain or plasmids | Description | Source or reference |
|---|---|---|
|
Strains |
|
|
|
E. coli DH5α |
Host for general cloning |
TransGen biotech |
|
E. coli ET-12567 |
Donor stain for conjugation between E. coli and S. spinosa |
[44] |
|
S.spinosa ATCC 49460 |
Wild strain |
ATCC |
|
S.spinosa 261P |
S.spinosa ATCC 49460 harboring pOJ261P |
This study |
|
Plasmid |
|
|
| pOJ260 |
E. coli–Streptomcyes shuttle vector; apr oriT repPUC lacZ |
[45] |
| pOJ261 |
pOJ260 with ermE* promoter |
Our lab |
| pOJ261P | pOJ260 with ermE*-controlled PntAB | This study |