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. 2014 Mar 14;13:40. doi: 10.1186/1475-2859-13-40

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Copyright © 2014 Liu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Metabolic flux distribution in B. subtilis BSSA/pHCMC04/pDG148-stu (a) and BSSA/pSAAroA/pDGSAAroD (b) during exponential growth phase. The estimated net fluxes were percentages of the relative rates normalized to the glucose uptake rates. Directions of net fluxes were represented by arrows. The gray arrows indicated flux related to biomass formation and fermentation products. The flux distributions were obtained from the best fit to the quantitative physiological data and the constraints derived from the MS measurements. F6P, fructose-6-phosphate; GAP, Glyceraldehyde 3-phosphate; AcCoA, acetyl-CoA; ICT, isocitrate; AKG, a-ketoglutarate; S7P, sedoheptulose-7-phosphate; DHS, 3-dehydroshikimic acid; SA, shikimic acid.