FIGURE 6.
Sphingolipid cross-sectional molecular area at 30 mN/m in the absence and presence of cholesterol. Each horizontal line corresponds to a different sphingolipid derivative (GalCers = filled symbols; SMs = unfilled symbols). The right-hand symbol represents the cross-sectional molecular area of the pure lipid in the absence of cholesterol; the left-hand symbol shows the condensed molecular area of each sphingolipid induced by either 0.5 (A) or 0.3 (B) mole fraction cholesterol. This latter area was calculated using eq 2 in Materials and Methods. The length of each horizontal line connecting paired symbols represents the change in sphingolipid molecular area, i.e. condensation, produced upon mixing with cholesterol: (○) 18:2 SM, (□) bovine SM, (△) egg SM, (◇) 16:0 SM, (▽) 18:0 SM, (bow tie) 18:1 SM, (☆) 24:1 SM, (●) 18:2 GalCer, (■) 18:1 GalCer, (▲) 24:1 GalCer, (▼) 18:0 GalCer, and (◆) 16:0 GalCer.