. Author manuscript; available in PMC: 2014 Apr 29.

Published in final edited form as: Biol Mass Spectrom. 1992 Oct;21(10):486–490. doi: 10.1002/bms.1200211004

Table 2.

Comparison of (1-¹³C)leucine enrichment in rat quadriceps muscle protein measured by capillary GC/combustion IRMS and standard preparative GC/ninhydrin IRMS

(1-¹³)Leucine infusion rate (mg 100 g⁻¹ h⁻¹	(1-¹³C)Leucine enrichment^a (GC/combustion IRMS)	(1-¹³C)Leucine enrichment^a (GC/ninhydrin IRMS)
0.10 for 6 h	0.0288 ± 0.0059 (20)	0.0256 ± 0.0003 (1.2)
0.20 for 6 h	0.0558 ± 0.0041 (7.3)	0.0560 ± 0.0007 (1.2)
0.40 for 6 h	0.1068 ± 0.0029 (2.7)	0.1042 ± 0.0104 (10)
0.64 for 8 h	0.1144 ± 0.0066 (5.8)	0.1094 ± 0.0012 (1.2)

Mean at.% excess ± SD for 4–6 injections on GC/combustion IRMS and 4 GC/ninhydrin IRMS analyses. Numbers in parentheses are the coefficients of variation.