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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Int J Biochem Cell Biol. 2014 Mar 1;50:112–122. doi: 10.1016/j.biocel.2014.02.019

Figure 6. Decreased content of partners of the NuRSERY complex in erythroblasts exposed for 4 days to EPO in combination with the class II-selective HDACi APHA9.

Figure 6

A) IP with anti-GATA1 antibodies probed with antibodies against GATA1, HDAC5 and EKLF of whole cell extracts from human proerythroblasts (0 h) or from erythroblasts exposed for 96 h to EPO either alone or with APHA9 (2 μM), as indicated. Results obtained with cells exposed to UBHA24 (2 μM) are reported for comparison. The same cells as those analyzed in Figure 5.

B) WB analysis with antibodies specific for GATA1, EKLF, pERK and ERK of whole cell extracts from human proerythroblasts (0 h) or from erythroblasts obtained after 96 h of exposure to EPO either alone or in combination with increasing concentration of APHA9 or UBHA24, as indicated. GAPDH was analyzed as loading control. The expected molecular weights are indicated on the left. The same cells as those analyzed in A.