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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Int J Biochem Cell Biol. 2014 Mar 1;50:112–122. doi: 10.1016/j.biocel.2014.02.019

Table I.

3H-Acetyl release activity of IP with HDAC1 and HDAC5 antibodies of lysates from human proerythroblasts.

HDAC1 (epu) HDAC5 (epu)
Day 10 ProE 11,474±275 7,729±520
+ EPO 24 h 13,082±470* 4,685±313*
+ EPO 48 h 14,448±807* 1,162±53*
HeLa cells (positive control) 7,888±25 n.d.
Negative control (IgG) 464±1 464±1

Results are normalized per cell number (extracts from 106 cells are analysed in each point) and are presented as mean (±SD) of three separate determinations. Similar results were obtained in an additional experiment.

*

p<0.01. n.d. = not done